Leeya Engel

EIPBN 2022

Invited Speaker

Leeya Engel

Stanford University 

Micropatterning of Electron Microscopy Grids for Improved Cellular Cryo-electron Tomography Throughput

Authors: Leeya Engel, Claudia G. Vasquez, Elisabeth A. Montabana, Belle M. Sow, Marcin P. Walkiewicz, William I. Weis and Alexander R. Dunn

Cryo-electron tomography enables nanoscale structural analysis of vitrified biological cells. Data acquisition currently suffers from low throughput, in part due to the low probability of positioning cells in imageable regions of EM grids. We address this limitation by applying photolithography to position cells and specific subcellular regions on EM grids.
Leeya Engel

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Ricardo Ruiz

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Michael Cullinan

Microscale Additive Manufacturing of Metal Interconnects Using Microscale Selective Laser Sintering
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